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Road Traffic Accidents (RTAs) results in significant public health issue in Nigeria, causing around 35,000 deaths annually. Globally, RTAs claim over one million lives each year, with 90% of fatalities occurring in low- and middle-income countries. This study investigates the factors contributing to RTAs in Osun State, Nigeria, using primary data from drivers, passengers, and other road users through structured questionnaires. Data from seven major cities were analyzed using descriptive statistics and chi-squared tests. Thirteen factors were found to be statistically significant in contributing to RTAs: poor road conditions, over-speeding, drunk driving, distracted driving, reckless driving, poor vehicle maintenance, overloading, fatigue, poor weather conditions, road design, lack of road safety education, weak enforcement of traffic laws, and poorly trained drivers. Factors like inadequate lighting, pedestrian behavior, and poor emergency response were not found to significantly contribute. The study recommends improving road infrastructure, focusing on road maintenance, filling potholes, resurfacing roads, and ensuring proper drainage to prevent further deterioration.