• 2021-11-10
• Agboola, T.D., Odeyemi O.
• Volume 3, Issue 2

Abstract

This study investigated the individual and combined antimicrobial activities of the methanolic extracts of Allium sativum bulbs and Garcinia kola seeds on selected bacterial isolates, assessed the minimum inhibitory concentrations of the crude extracts with a view to determine if the crude extracts have synergistic effect. The seeds of Garcinia kola and bulbs of Allium sativum were purchased from Central Market, Ile-Ife Southwest Nigeria, dried, and ground into fine powder separately. The extract of ground plant materials were obtained by soaking in 60% methanol separately, filtered, concentrated in vacuo, lyophilized and screened for phytochemical components and antibacterial activities individually and in combined form against some selected bacterial isolates. Minimum inhibitory concentrations (MIC) assay of plant extracts and their combinations were performed. Phytochemical screening revealed the presence of Saponins, Tannins, Flavonoids, reducing sugars, Alkaloids and Steroids in G. kola while Saponins, Triterpenes and Steroids were present in A. sativum. The plant extracts exhibited different degrees of antimicrobial activities against the selected bacteria and synergistic effect against some tested pathogens. The MIC of the methanolic extract of G. kola ranges from 0.313 mg/ml to 2.5 mg/ml, MIC exhibited by A. sativum was 2.5 mg/ml while MIC that ranged from 0.313 mg/ml and 1.25 mg/ml was observed when the extracts were combined at equal proportion. The study concluded that crude extracts of G. kola and A. sativum exhibited potentials of synergy against pathogenic bacteria which can be explored in providing solutions to multidrug resistance.